Integration of transcriptome and proteome analysis reveals the mechanism of freezing tolerance in winter rapeseed

Winter rapeseed seedlings are susceptible to low temperature during overwintering in Northwest China, leading reduced crops production. Freezing stress is one of the main environmental stresses China from late autumn early spring, an eventful period for overwinter survival rate winter rapeseed. However, molecular mechanism freezing tolerance formation still very backward In this study, using a pair freezing-sensitive and freezing-resistant cultivars NQF24 NTS57, exhaustive effects on were evaluated by analyzing leaf at levels transcriptome, proteome, physiology ultrastructure. There 8497 7358 differentially expressed genes (DEGs) 418 573 abundant proteins (DAPs) identified NTS57 under stress, respectively. Function enrichment analysis showed that most enriched DEGs DAPs associated with plant hormones signal transduction, fatty acid metabolism, ribosome, plant-pathogen interaction secondary metabolites biosynthesis. formed enhanced signals increased biosynthesis protein metabolites, reactive oxygen species (ROS) scavenging, more osmolytes, lower lipid peroxidation, stronger cell stability. These results can be taken as selection indicators breeding program